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102 Z. Rechtsmedizin 1 (1989)

handle is hein.journals/injlegame102 and id is 1 raw text is: Zeitschrift fur Z Rechtsmed (1989) 102:1-4 Rechtsmedizin © Springer-Verlag 1989 Original works Determination of methadone in human hair by radioimmunoassay S.Balabanoval and H. U. Wolf2 1Department of Forensic Medicine, University of Ulm, Prittwitzstrasse 6, D-7900 Ulm, Federal Republic of Germany 2Department of Pharmacology and Toxicology, University of Ulm, Oberer Eselsberg, D-7900 Ulm, Federal Republic of Germany Summary. The concentrations of methadone in human hair were measured. The washed hair was cut in 1-mm pieces approximately, then incubated overnight at 45°C with 0.1 m HCl. The extracts were alcalized by 1 m NaOH and diluted by phosphate buffer, pH 7.4. The methadone concentrations were determined by radioimmunoassay. The method is simple, rapid, and practicable for routine determination. Key word: Methadone, radioimmunologic determination in hair Zusammenfassung. Die Konzentration von Methadon im menschlichen Haar wurde bestimmt. Die gewaschenen Haarproben wurden in ca. 1 mm lange Stucke geschnitten und mit 0.1 m HCl uber Nacht bei 45°C inkubiert. Die Extrakte wurden mit 1 m NaOH alkalisiert und mit Phosphat-Puffer pH 7.4 verdiinnt. Die Methadonkonzentrationen wurden mittels Radioimmuno- assay gemessen. Diese Methode stellt eine einfache und schnelle Routine- bestimmung dar. Schlisselwort: Methadon, radioimmunologische Bestimmung im Haar Introduction Methadone is a synthetic long-acting analgesic promoted as a treatment in de- toxification and maintenance programs of heroin addiction (Isbell and Vogell 1949; Dole and Nyswandes 1965). However, methadone use results also in habit formation (Isbell and Vogell 1949; Symonds 1977; Ettinger et al. 1979). Con- sequently, the use of methadone represents a serious problem and indicates the necessity of control. In this study, we describe the determination of methadone in human hair by radioimmunoassay. Offprint requests to: S. Balabanova